Difference between revisions of "Part:BBa K902048"
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| − | + | XylE codes for the extra-diol dioxygenase called catechol 2,3-dioxygenase<sup>1</sup>. This enzyme converts catechol to 2-hydroxymuconic semialdehyde, by cleaving catechol adjacent to the two hydroxyl groups<sup>1</sup>.Because catechol is colourless and 2-HMS is a yellow compound visual assays can be used to monitor the activity of XylE. The tetR promoter (R0040) was used instead of the glucose repressible cstA promoter (K118011) so that the transformed cells could be grown in media containing glucose and XylE could still be expressed. | |
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Latest revision as of 04:32, 3 October 2012
Constitutive XylE generator
XylE codes for the extra-diol dioxygenase called catechol 2,3-dioxygenase1. This enzyme converts catechol to 2-hydroxymuconic semialdehyde, by cleaving catechol adjacent to the two hydroxyl groups1.Because catechol is colourless and 2-HMS is a yellow compound visual assays can be used to monitor the activity of XylE. The tetR promoter (R0040) was used instead of the glucose repressible cstA promoter (K118011) so that the transformed cells could be grown in media containing glucose and XylE could still be expressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 399
Illegal NgoMIV site found at 571
Illegal AgeI site found at 922 - 1000COMPATIBLE WITH RFC[1000]