Difference between revisions of "Part:BBa K902067"
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| + | NOTE: This was our original submission of the part. The <i>S7 micrococcal nuclease</i> contains a mutation at a single nucleotide and is thus ineffective. We have since resubmitted the part as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902084 K902084] in which we repaired the mutation. Please view the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902084 K902084] page and our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch Wiki] for details about the system. | ||
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| + | This system consists of the rhamnose promoter, a strong ribosomal binding site, and S7 micrococcal nuclease. This is part of the final kill system which is repressed by glucose. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 22:18, 28 October 2012
Rhamnose promoter with S7 micrococal nuclease
NOTE: This was our original submission of the part. The S7 micrococcal nuclease contains a mutation at a single nucleotide and is thus ineffective. We have since resubmitted the part as K902084 in which we repaired the mutation. Please view the K902084 page and our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch Wiki] for details about the system.
This system consists of the rhamnose promoter, a strong ribosomal binding site, and S7 micrococcal nuclease. This is part of the final kill system which is repressed by glucose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]