Difference between revisions of "Part:BBa K902062"

 
 
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<html>CAR (carboxylic acid reductase) is a gene present in <i>Nocardia iowensis</i> that is an ATP/NADPH-dependent carboxylic acid reductase. It is highly non-specific and able to reduce a range of carboxylic acids. It is dependent on <a href="https://parts.igem.org/Part:BBa_K902061"><i>NPT</i></a> for full function.</html>
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[[Image:Ucalgary Decarboxylation Team CAR Mechanism.jpg|center|thumb|Taken from He <i>et al</i>, 2004.|500px]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:50, 4 October 2012

CAR (Nocardia carboxylic acid reductase)

CAR (carboxylic acid reductase) is a gene present in Nocardia iowensis that is an ATP/NADPH-dependent carboxylic acid reductase. It is highly non-specific and able to reduce a range of carboxylic acids. It is dependent on NPT for full function.

Taken from He et al, 2004.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1302
    Illegal NotI site found at 1691
    Illegal NotI site found at 2452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1016
    Illegal BglII site found at 1448
    Illegal BglII site found at 1619
    Illegal BglII site found at 2102
    Illegal XhoI site found at 1498
    Illegal XhoI site found at 1582
    Illegal XhoI site found at 2509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3108
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 256
    Illegal BsaI.rc site found at 1402
    Illegal BsaI.rc site found at 1798