Difference between revisions of "Part:BBa K902040:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K902040 short</partinfo>
 
<partinfo>BBa_K902040 short</partinfo>
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===Design Notes===
 
===Design Notes===
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none
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<html><p>
  
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Please refer to <a href="https://parts.igem.org/Part:BBa_K902034:Design">Part Design</a> page for <i>carAd</i>.
  
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</p></html>
  
 
===Source===
 
===Source===
  
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no
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<i>carAd</i> gene was amplified by PCR using <i>Pseudomonas putida</i> plasmid pCAR1.2 as a template. B0034 was transformed from the parts registry.
  
 
===References===
 
===References===
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Shintani,M., Yoshida,T., Habe,H., Omori,T. and Nojiri,H. Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria. Appl. Microbiol. Biotechnol. 67 (3), 370-382 (2005).

Latest revision as of 02:52, 4 October 2012

B0034 + CarAd


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 81
    Illegal SapI.rc site found at 69


Design Notes

Please refer to Part Design page for carAd.

Source

carAd gene was amplified by PCR using Pseudomonas putida plasmid pCAR1.2 as a template. B0034 was transformed from the parts registry.

References

Shintani,M., Yoshida,T., Habe,H., Omori,T. and Nojiri,H. Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria. Appl. Microbiol. Biotechnol. 67 (3), 370-382 (2005).