Difference between revisions of "Part:BBa K842001"

 
 
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<partinfo>BBa_K842001 short</partinfo>
 
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[[Image:USC_flhd.gif|300px|right]]
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flhD along with flhC forms the master regulation operon for the synthesis of flagella in E. coli.  They are responsible for the downstream transcription of the class 2 flagella genes.  flhD is directly induced by several separate systems, among these is the two-component quorum sensing response system, qseB and qseC.
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flhD along with flhC form the master regulation operon for the synthesis of flagella in E. coli.  They are responsible for the downstream transcription of the class 2 flagella genes.  flhD is directly induced by several separate systems; among these is the two-component quorum sensing response system, qseB and qseC.
  
 
Used for reconstitution of flagella apparatus in E. coli B strains.
 
Used for reconstitution of flagella apparatus in E. coli B strains.
  
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Cloning and Uses
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Cloning and Uses:
 
This part was generated via PCR from the E. coli strain DH5&#945; and cloned into the vector pSB1C3. FlhD is to be used in conjunction with flhC in order to form the transcriptional operon for the production of flagella.  This part can be used either to generate a flagella in an E. coli strain in which they are not already present, or to promote further formation of pre-existing flagella.
 
This part was generated via PCR from the E. coli strain DH5&#945; and cloned into the vector pSB1C3. FlhD is to be used in conjunction with flhC in order to form the transcriptional operon for the production of flagella.  This part can be used either to generate a flagella in an E. coli strain in which they are not already present, or to promote further formation of pre-existing flagella.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 01:04, 4 October 2012

flhD

USC flhd.gif

flhD along with flhC form the master regulation operon for the synthesis of flagella in E. coli. They are responsible for the downstream transcription of the class 2 flagella genes. flhD is directly induced by several separate systems; among these is the two-component quorum sensing response system, qseB and qseC.

Used for reconstitution of flagella apparatus in E. coli B strains.

Cloning and Uses: This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. FlhD is to be used in conjunction with flhC in order to form the transcriptional operon for the production of flagella. This part can be used either to generate a flagella in an E. coli strain in which they are not already present, or to promote further formation of pre-existing flagella.








Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]