Difference between revisions of "Part:BBa K799028"

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<partinfo>BBa_K799028 short</partinfo>
 
<partinfo>BBa_K799028 short</partinfo>
  
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GFP modified to remove all BsaI sites.
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GFP coding sequence in which naturally occurring BsaI sites have been re-coded.  The encoded protein sequence remains unchanged and works as expected.
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This GFP coding sequence (CDS) is compatible with BsaI-dependent assembly strategies, such as that described in RFC88 - Yeast Golden Gate.  
  
 
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[[Image:GFP no bsa sequencing.png]]
 
[[Image:GFP no bsa sequencing.png]]
  
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This is the sequencing result for human codon optimized GFP. As can be seen, it is a perfect match to our expected sequence.
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This is the sequencing result for BsaI-less GFP coding sequence. As can be seen, it is a perfect match to our expected sequence.

Latest revision as of 03:41, 3 October 2012

typeIIS-less GFP

GFP coding sequence in which naturally occurring BsaI sites have been re-coded. The encoded protein sequence remains unchanged and works as expected.

This GFP coding sequence (CDS) is compatible with BsaI-dependent assembly strategies, such as that described in RFC88 - Yeast Golden Gate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Map

GFP bsa- plasmid.png

Sequencing

GFP no bsa sequencing.png

This is the sequencing result for BsaI-less GFP coding sequence. As can be seen, it is a perfect match to our expected sequence.