Difference between revisions of "Part:BBa K732002"

 
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<partinfo>BBa_K732002 short</partinfo>
 
<partinfo>BBa_K732002 short</partinfo>
  
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This construct contains a strong constitutive promoter (J23119) as well as an RBS (B0034) upstream of synCG-&#946; (K732001), and should strongly express a synthetic version of the beta subunit of human chorionic gonadotropin.
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Human chorionic gonadotropin (hCG) is a hormone produced during pregnancy. Its beta subunit (hCG-β) contains epitopes recognized by pregnancy tests. The full hCG dimer is also used as an ovulation inducer and can stimulate the production of testosterone in men.
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This sequence could ideally be used to express hCG for any downstream uses, and also as an output signal since it could trigger pregnancy tests at low concentrations when properly folded.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:03, 1 October 2013

synCG-β + Promoter + RBS

Human chorionic gonadotropin (hCG) is a hormone produced during pregnancy. Its beta subunit (hCG-β) contains epitopes recognized by pregnancy tests. The full hCG dimer is also used as an ovulation inducer and can stimulate the production of testosterone in men.

This sequence could ideally be used to express hCG for any downstream uses, and also as an output signal since it could trigger pregnancy tests at low concentrations when properly folded.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 224
    Illegal NgoMIV site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]