Difference between revisions of "Part:BBa K917002:Design"

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===Design Notes===
 
===Design Notes===
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none
+
−
 
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The lac promoter and Red Fluorescent Protein (RFP) from BBa_J04450 (but without the terminator) have been added to SacB (BBa_K322921).
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+
  
 
===Source===
 
===Source===
  
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From Bacillus subtilis genomic DNA
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From ''Bacillus subtilis'' genomic DNA
  
 
===References===
 
===References===
 
Gay, P., Coq, D. l., Strinmetz, M., Ferrari, E., & Hoch, J. A. (1983). Cloning Structural Gene SacB, which Codes for Exoenzyme Levansucrase of Bacillus subtilis: Expression of the Gene in Esherichia coli. Journal of Bacteriology , 1424-1431.  
 
Gay, P., Coq, D. l., Strinmetz, M., Ferrari, E., & Hoch, J. A. (1983). Cloning Structural Gene SacB, which Codes for Exoenzyme Levansucrase of Bacillus subtilis: Expression of the Gene in Esherichia coli. Journal of Bacteriology , 1424-1431.  
 
French, C., & Kowal, M. (2010, 09 24). B. subtilis levansucrase. Lethal to E.coli in presence of sucrose. Retrieved 2012, from Registry of standard biological parts: https://parts.igem.org/Part:BBa_K322921
 
French, C., & Kowal, M. (2010, 09 24). B. subtilis levansucrase. Lethal to E.coli in presence of sucrose. Retrieved 2012, from Registry of standard biological parts: https://parts.igem.org/Part:BBa_K322921

Latest revision as of 20:53, 1 October 2012

lac promoter plus RFP and sacB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lac promoter and Red Fluorescent Protein (RFP) from BBa_J04450 (but without the terminator) have been added to SacB (BBa_K322921).

Source

From Bacillus subtilis genomic DNA

References

Gay, P., Coq, D. l., Strinmetz, M., Ferrari, E., & Hoch, J. A. (1983). Cloning Structural Gene SacB, which Codes for Exoenzyme Levansucrase of Bacillus subtilis: Expression of the Gene in Esherichia coli. Journal of Bacteriology , 1424-1431. French, C., & Kowal, M. (2010, 09 24). B. subtilis levansucrase. Lethal to E.coli in presence of sucrose. Retrieved 2012, from Registry of standard biological parts: https://parts.igem.org/Part:BBa_K322921