Difference between revisions of "Part:BBa J45006"

(2020 XHD-ShanDong-China’s Contribution)
 
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<partinfo>BBa_J45006 short</partinfo>
 
<partinfo>BBa_J45006 short</partinfo>
  
From S. cerevisiae (yeast)
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Alcohol acetyltransferase I (ATFI) encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols. It was obtained from ''S. cerevisiae''.
  
 
===Usage and Biology===
 
===Usage and Biology===
*Gene function
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We will be interested in using AATase I to catalyze the reaction: isoamyl alcohol -> isoamyl acetate. Isoamyl acetate is a volatile ester with a strong banana-like scent. (The related enzyme AATase II, which is encoded by the gene ATF2, performs a similar function...but it is less efficient.)
The gene ATF1 encodes the enzyme alcohol acetyltransferase 1, which produces acetate esters from acetyl CoA and alcohols.  We will be interested in using ATF1 to catalyze the reaction of isoamyl alcohol -> isoamyl acetate; the product is of interest because it is a volatile ester with a strong banana-like scent.
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*Horton et al. reported a yield of 1.8mM isoamyl acetate 40 hours after the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene.
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According to studies done by [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12937998&query_hl=16&itool=pubmed_docsum Horton et al.], the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene yields 1.8mM isoamyl acetate after 40 hours.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_J45006 parameters</partinfo>
 
<partinfo>BBa_J45006 parameters</partinfo>
 
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===<h1>2020 XHD-ShanDong-China’s Contribution</h1>===
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<h2> Introduction </h2>
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The acetyltransferase ATF1 one of three known S. cerevisiae alcohol acetyl transferases responsible for the synthesis of volatile esters. In this paper, ATF1 is proved to also acetylate alcohols to make various acetates, which are the main component of moth pheromones. Therefore, we could use this enzyme ATF1 to produce moth pheromone compounds biologically, which could be used in pest control.
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<h2>Results </h2>
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In this paper, They decided to determine whether this enzyme was responsible for the background formation of acetates when supplemented with long-chain fatty alcohols. First, They added 19 different fatty alcohol substrates to the atf1∆ knockout strain. The atf1∆ knockout strain produced barely detect- able quantities of any long-chain acetates, suggesting that ATF1 is the only yeast enzyme with significant activity towards fatty alcohols of C10 and longer (Fig. 2). To compare the capacity of ATF1 to that of EaDAcT to produce acetates, we expressed either enzyme in an atf1∆ knockout strain and incubated the cultures with the fatty alcohol sub- strates. Quantification of the acetates formation provided additional evidence that ATF1 can convert a wide range of fatty alcohols into their corresponding acetates (Fig. 2). Further, the levels of acetates formed in ATF1-expressing yeast were 10–40 times higher than those in yeast express- ing EaDAcT, except for 16:OH, 18:OH, and Z9-18:OH which were also poor substrates for ATF1 (Fig. 2).
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<li style="display: inline-block;"> [[File:T--XHD-Shandong-China--literature_figure_1.png|thumb|none|800px]]
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<h2>Reference</h2>
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Ding, Bao‑Jian, et al. “The Yeast ATF1 Acetyltransferase Efficiently Acetylates Insect Pheromone Alcohols: Implications for the Biological Production of Moth Pheromones.” Lipids, vol. 51, no. 4, 2016, pp. 469–475.

Latest revision as of 06:48, 22 October 2020

alcohol acetyltransferase I (ATF1)

Alcohol acetyltransferase I (ATFI) encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols. It was obtained from S. cerevisiae.

Usage and Biology

We will be interested in using AATase I to catalyze the reaction: isoamyl alcohol -> isoamyl acetate. Isoamyl acetate is a volatile ester with a strong banana-like scent. (The related enzyme AATase II, which is encoded by the gene ATF2, performs a similar function...but it is less efficient.)

According to studies done by [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12937998&query_hl=16&itool=pubmed_docsum Horton et al.], the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene yields 1.8mM isoamyl acetate after 40 hours.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534


2020 XHD-ShanDong-China’s Contribution

Introduction

The acetyltransferase ATF1 one of three known S. cerevisiae alcohol acetyl transferases responsible for the synthesis of volatile esters. In this paper, ATF1 is proved to also acetylate alcohols to make various acetates, which are the main component of moth pheromones. Therefore, we could use this enzyme ATF1 to produce moth pheromone compounds biologically, which could be used in pest control.

Results

In this paper, They decided to determine whether this enzyme was responsible for the background formation of acetates when supplemented with long-chain fatty alcohols. First, They added 19 different fatty alcohol substrates to the atf1∆ knockout strain. The atf1∆ knockout strain produced barely detect- able quantities of any long-chain acetates, suggesting that ATF1 is the only yeast enzyme with significant activity towards fatty alcohols of C10 and longer (Fig. 2). To compare the capacity of ATF1 to that of EaDAcT to produce acetates, we expressed either enzyme in an atf1∆ knockout strain and incubated the cultures with the fatty alcohol sub- strates. Quantification of the acetates formation provided additional evidence that ATF1 can convert a wide range of fatty alcohols into their corresponding acetates (Fig. 2). Further, the levels of acetates formed in ATF1-expressing yeast were 10–40 times higher than those in yeast express- ing EaDAcT, except for 16:OH, 18:OH, and Z9-18:OH which were also poor substrates for ATF1 (Fig. 2).


  • T--XHD-Shandong-China--literature figure 1.png

    Reference

    Ding, Bao‑Jian, et al. “The Yeast ATF1 Acetyltransferase Efficiently Acetylates Insect Pheromone Alcohols: Implications for the Biological Production of Moth Pheromones.” Lipids, vol. 51, no. 4, 2016, pp. 469–475.