Difference between revisions of "Part:BBa K942012:Design"
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===References=== | ===References=== | ||
| − | Kristiansen, E. et all (2011) Structural characteristics of a novel antifreeze protein from the longhorn beetle Rhagium inquisitor. Insect Biochem Mol Biol. 41(2):109-17. | + | Kristiansen, E. et all (2011) Structural characteristics of a novel antifreeze protein from the longhorn beetle ''Rhagium inquisitor''. Insect Biochem Mol Biol. 41(2):109-17. |
Latest revision as of 23:15, 30 September 2012
proU+RiAFP+Term
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 951
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The RiAFP is expressed when around 300 mM NaCl added. This part was designed by adding a 6xHis tag for affinity purification to the anti freeze protein from Raghium inquisitor. This part was codon optimized for its expression in E.coli.
Source
The Raghium inquisitor Anti Freeze Protein (RiAFP) is a 137 AA protein that prevents the growth of ice crystals by adsorption to their surface. The proU promoter is from E.coli and works activating transcription when the bacterium is osmotically stressed. (Kristiansen E, 2011)
References
Kristiansen, E. et all (2011) Structural characteristics of a novel antifreeze protein from the longhorn beetle Rhagium inquisitor. Insect Biochem Mol Biol. 41(2):109-17.