Difference between revisions of "Part:BBa K864150"
 
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[[Image:Cat-SacB.png | 600px]] <br>
 
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''E. coli strain carrying a chromosomally integrated copy of cat-sacB (<partinfo>BBa_K864150</partinfo>) plated on a sucrose plate (A) and on a chloramphenicol plate (B). E. coli wildtype strain MG1655 was used as a control.''
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'''iGEM12_Uppsala_University:''' ''E. coli strain carrying a chromosomally integrated copy of cat-sacB (<partinfo>BBa_K864150</partinfo>) plated on a sucrose plate (A) and on a chloramphenicol plate (B). E. coli wildtype strain MG1655 was used as a control.''
 
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Latest revision as of 20:32, 30 September 2012

Cat-SacB cassette for scarless chromosomal knockout

Cat-SacB

Consists of chloramphenicol resistance marker BBa_P1004 and the Bacillus subtilis levansucrase SacB BBa_K322921

Usage and Biology

Cat-SacB.png

iGEM12_Uppsala_University: E. coli strain carrying a chromosomally integrated copy of cat-sacB (BBa_K864150) plated on a sucrose plate (A) and on a chloramphenicol plate (B). E. coli wildtype strain MG1655 was used as a control.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters