Difference between revisions of "Part:BBa K845005:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | We used an overlapping PCR to assemble the sequences. | ||
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| + | Our safety sheet: https://static.igem.org/mediawiki/2012/5/53/TapZ.pdf | ||
===Source=== | ===Source=== | ||
| − | . | + | Tap and EnvZ were extracted from BW25133 <i>E. Coli</i>. |
===References=== | ===References=== | ||
Latest revision as of 17:20, 30 September 2012
TapZ engineered membrane receptor
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used an overlapping PCR to assemble the sequences.
Our safety sheet: https://static.igem.org/mediawiki/2012/5/53/TapZ.pdf
Source
Tap and EnvZ were extracted from BW25133 E. Coli.
References
Manson MD, Blank V, Brade G, Higgins CF.Peptide chemotaxis in E. coli involves the Tap signal transducer and the dipeptide permease. Nature. 1986 May 15-21;321(6067):253-6.
Maeda S, Takayanagi K, Nishimura Y, Maruyama T, Sato K, Mizuno T. Activation of the osmoregulated ompC gene by the OmpR protein in Escherichia coli: a study involving synthetic OmpR-binding sequences. J Biochem. 1991 Sep;110(3):324-7.
Weerasuriya S, Schneider BM, Manson MD. Chimeric chemoreceptors in Escherichia coli: signaling properties of Tar-Tap and Tap-Tar hybrids. J Bacteriol. 1998 Feb;180(4):914-20.
Cai SJ, Inouye M. EnvZ-OmpR interaction and osmoregulation in Escherichia coli. J Biol Chem. 2002 Jul 5;277(27):24155-61. Epub 2002 Apr 24.
Baumgartner JW, Kim C, Brissette RE, Inouye M, Park C, Hazelbauer GL. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of osmosensor EnvZ. J Bacteriol. 1994 Feb;176(4):1157-63.