Difference between revisions of "Part:BBa K741008"

Latest revision as of 01:17, 3 October 2012

pCon(0.244)-RBS-crogfp-T

Here, pCon(J23114) constantly expressing the fusion protein crogfp(BBa_K741006) at 10% of the strength of pCon(J23100).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 124
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 918

Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).

Data

pCon(0.244)-RBS-crogfp-T
	OD			fluorescence intensity
t/min	Parallel	Experimental group	M9 medium	Parallel	Experimental group	M9 medium
0	1	0.136	0.0559	1	132.355	18.095
	2	0.1313		2	116.709
45	1	0.1988	0.0522	1	180.537	19.878
	2	0.2004		2	172.497
90	1	0.3153	0.0543	1	267.399	21.225
	2	0.3126		2	243.579
135	1	0.445	0.0541	1	346.814	20.907
	2	0.4478		2	357.696
180	1	0.4949	0.0533	1	423.673	21.545
	2	0.5014		2	454.136

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K741008 short</partinfo>
-Here, pCon(J23114) constantly expressing the fusion protein crogfp at 10% of the strength of pCon(J23100).
+Here, pCon(J23114) constantly expressing the fusion protein crogfp(BBa_K741006) at 10% of the strength of pCon(J23100).
 <!-- Add more about the biology of this part here
@@ Line 19: / Line 19: @@
   <tr height=19 style='height:14.25pt'>
    <td colspan=7 height=19 class=xl24 width=742 style='height:14.25pt;
-   width:556pt'>pCon(0.244)-RBS-crogfp-T</td>
+   width:556pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;pCon(0.244)-RBS-crogfp-T</td>
   </tr>
   <tr height=19 style='height:14.25pt'>
@@ Line 28: / Line 28: @@
   <tr height=19 style='height:14.25pt'>
    <td height=19 class=xl25 style='height:14.25pt;border-top:none'>t/min</td>
-   <td class=xl25 style='border-top:none;border-left:none' x:str="Parellel ">Parellel<span
+   <td class=xl25 style='border-top:none;border-left:none' x:str="Parallel ">Parallel<span
    style='mso-spacerun:yes'>&nbsp;</span></td>
    <td class=xl25 style='border-top:none;border-left:none'>Experimental group</td>
    <td class=xl25 style='border-top:none;border-left:none'>M9 medium</td>
-   <td class=xl25 style='border-top:none;border-left:none' x:str="Parellel ">Parellel<span
+   <td class=xl25 style='border-top:none;border-left:none' x:str="Parallel ">Parallel<span
    style='mso-spacerun:yes'>&nbsp;</span></td>
    <td class=xl25 style='border-top:none;border-left:none'>Experimental group</td>