Difference between revisions of "Part:BBa K741003"

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<partinfo>BBa_K741003 short</partinfo>
 
<partinfo>BBa_K741003 short</partinfo>
  
−
Express GFP only if protein CI activated the promoter pRM.
+
 
 +
Promoter pRM with GFP gene downstream. The pRM is a modified one, whose OR3 (the third right operator) is mutated to be obliterated.
 +
This means our pRM is relatively difficult to be repressed by Cro dimer and can always be activated by CI dimer. We use this part to test the basic level of expression of modified pRM.
 +
 
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 11: Line 15:
 
<partinfo>BBa_K741003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K741003 SequenceAndFeatures</partinfo>
 
<div class="pic">
 
<div class="pic">
−
[[Image:USTC2012Result2.png]]
+
[[Image:USTC2012Result2_副本.png]]
 
<p>Figure 2 shows that the fluorescence intensity of prm-gfp is so weak in comparison with plac-gfp that we can assume that the leak expression of prm is very few, which doesn't affect our experiments.</p>
 
<p>Figure 2 shows that the fluorescence intensity of prm-gfp is so weak in comparison with plac-gfp that we can assume that the leak expression of prm is very few, which doesn't affect our experiments.</p>
 +
 +
==Data==
 +
 +
 +
<table border=1>
 +
 +
<tr height=19 style='height:14.25pt'>
 +
  <td colspan=4 height=19 class=xl26 width=386 style='height:14.25pt;
 +
  width:290pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;pRM-RBS-GFP-T</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td colspan=4 height=19 class=xl26 style='height:14.25pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;OD</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>t/min</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>Parallel</td>
 +
  <td class=xl26 style='border-top:none;border-left:none'>Experimental group</td>
 +
  <td class=xl28 style='border-top:none;border-left:none'>M9 medium</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>0</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="5.9299999999999999E-2">0.0593</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="4.4999999999999998E-2">0.045</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="6.4199999999999993E-2">0.0642</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="6.7799999999999999E-2">0.0678</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>45</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="6.0499999999999998E-2">0.0605</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="4.36E-2">0.0436</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="7.3400000000000007E-2">0.0734</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="6.2E-2">0.062</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>90</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="6.4799999999999996E-2">0.0648</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="4.3499999999999997E-2">0.0435</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="7.3499999999999996E-2">0.0735</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="7.0199999999999999E-2">0.0702</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>135</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="8.1100000000000005E-2">0.0811</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="4.4699999999999997E-2">0.0447</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="7.1599999999999997E-2">0.0716</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="6.7699999999999996E-2">0.0677</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>180</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="7.2300000000000003E-2">0.0723</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="4.4600000000000001E-2">0.0446</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="8.2799999999999999E-2">0.0828</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="7.8899999999999998E-2">0.0789</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 colspan=4 class=xl29 style='height:14.25pt;mso-ignore:colspan'></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td colspan=4 height=19 class=xl26 style='height:14.25pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;pRM-RBS-GFP-T</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td colspan=4 height=19 class=xl26 style='height:14.25pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;fluorescence
 +
  intensity</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>t/min</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>Parallel</td>
 +
  <td class=xl26 style='border-top:none;border-left:none'>Experimental group</td>
 +
  <td class=xl28 style='border-top:none;border-left:none'>M9 medium</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>0</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>19.55</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="11.801">11.801</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="19.571000000000002">19.571</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="19.352">19.352</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>45</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="21.547999999999998">21.548</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="13.385999999999999">13.386</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="20.504999999999999">20.505</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="19.106999999999999">19.107</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>90</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="24.440999999999999">24.441</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="11.541">11.541</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="23.777000000000001">23.777</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="24.503">24.503</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>135</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="28.937999999999999">28.938</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="11.894">11.894</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="27.884">27.884</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>27.81</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none' x:num>180</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="41.375999999999998">41.376</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="11.066000000000001">11.066</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num="39.573">39.573</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl27 style='border-top:none;border-left:none' x:num>3</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'
 +
  x:num="39.795000000000002">39.795</td>
 +
  <td class=xl27 style='border-top:none;border-left:none'>　</td>
 +
</tr>
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K741003 parameters</partinfo>
 
<partinfo>BBa_K741003 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 19:37, 2 October 2012

pRM-RBS-GFP-T


Promoter pRM with GFP gene downstream. The pRM is a modified one, whose OR3 (the third right operator) is mutated to be obliterated. This means our pRM is relatively difficult to be repressed by Cro dimer and can always be activated by CI dimer. We use this part to test the basic level of expression of modified pRM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752

USTC2012Result2 副本.png

Figure 2 shows that the fluorescence intensity of prm-gfp is so weak in comparison with plac-gfp that we can assume that the leak expression of prm is very few, which doesn't affect our experiments.

Data

                      pRM-RBS-GFP-T
                          OD
t/min Parallel Experimental group M9 medium
0 1 0.0593 0.045
　 2 0.0642 　
　 3 0.0678 　
45 1 0.0605 0.0436
　 2 0.0734 　
　 3 0.062 　
90 1 0.0648 0.0435
　 2 0.0735 　
　 3 0.0702 　
135 1 0.0811 0.0447
　 2 0.0716 　
　 3 0.0677 　
180 1 0.0723 0.0446
　 2 0.0828 　
　 3 0.0789 　
                       pRM-RBS-GFP-T
                  fluorescence intensity
t/min Parallel Experimental group M9 medium
0 1 19.55 11.801
　 2 19.571 　
　 3 19.352 　
45 1 21.548 13.386
　 2 20.505 　
　 3 19.107 　
90 1 24.441 11.541
　 2 23.777 　
　 3 24.503 　
135 1 28.938 11.894
　 2 27.884 　
　 3 27.81 　
180 1 41.376 11.066
　 2 39.573 　
　 3 39.795