Difference between revisions of "Part:BBa K779316"

Latest revision as of 20:49, 29 September 2012

3' Hammerhead with loss-of-function mutation MammoBlock

This sequence, along with Part:BBa_K779318, is designed to test the functionality of a hammerhead ribozyme in vitro. Hammerhead ribozymes are self-cutting strands of catalytic RNA. After a hammerhead ribozyme is transcribed in a cell, it folds into a secondary structure, which causes self-cleavage. Because of their RNA-cleaving function, hammerheads may be used to regulate protein expression and to make other RNA parts. For more information on hammerheads in general, see http://en.wikipedia.org/wiki/Hammerhead_ribozyme .

This sequence is designed for in vitro transcription - a promoter and terminator corresponding to the in vitro transcription kit should be attached via PCR. After IVT, the RNA transcript should be analyzed on a gel, to determine whether cleavage occurred.

This sequence is called a 3' hammerhead because its cut site is near its 3' end. The corresponding loss-of-site mutation, which should not cleave, serves as a control.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4
Illegal BsaI.rc site found at 178

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 __NOTOC__
 <partinfo>BBa_K779316 short</partinfo>
-To be completed by MIT iGEM 2012.
+This sequence, along with [[Part:BBa_K779318]], is designed to test the functionality of a hammerhead ribozyme in vitro.  Hammerhead ribozymes are self-cutting strands of catalytic RNA. After a hammerhead ribozyme is transcribed in a cell, it folds into a secondary structure, which causes self-cleavage. Because of their RNA-cleaving function, hammerheads may be used to regulate protein expression and to make other RNA parts. For more information on hammerheads in general, see http://en.wikipedia.org/wiki/Hammerhead_ribozyme .
+This sequence is designed for in vitro transcription - a promoter and terminator corresponding to the in vitro transcription kit should be attached via PCR.  After IVT, the RNA transcript should be analyzed on a gel, to determine whether cleavage occurred.
+This sequence is called a 3' hammerhead because its cut site is near its 3' end.  The corresponding loss-of-site mutation, which should not cleave, serves as a control.
 <!-- Add more about the biology of this part here