Difference between revisions of "Part:BBa K779301"
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<partinfo>BBa_K779301 short</partinfo> | <partinfo>BBa_K779301 short</partinfo> | ||
− | + | An engineered variant of the original green fluorescent protein from ''Aequorea victoria'' that fluoresces yellow. | |
+ | |||
+ | <b> Testing eYFP driven by a Hef1A promoter (BBa_K779200) </b> | ||
+ | <br> | ||
+ | [[Image:EYFPchar.png]] | ||
+ | <br> | ||
+ | <i> This image was taken on an Evos microscope at 10X. On the left, there is a brightfield image of 200,000 cells transiently transfected with Lipofectamine 2000 with 500 ng of Hef1A:eYFP. On the right, is an image taken in the FITC channel and we see high transfection efficiency and eYFP expression 48 hours post-transfection. </i> | ||
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Latest revision as of 02:23, 11 October 2012
eYFP MammoBlock
An engineered variant of the original green fluorescent protein from Aequorea victoria that fluoresces yellow.
Testing eYFP driven by a Hef1A promoter (BBa_K779200)
This image was taken on an Evos microscope at 10X. On the left, there is a brightfield image of 200,000 cells transiently transfected with Lipofectamine 2000 with 500 ng of Hef1A:eYFP. On the right, is an image taken in the FITC channel and we see high transfection efficiency and eYFP expression 48 hours post-transfection.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 211
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 211
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 211
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 211
- 1000COMPATIBLE WITH RFC[1000]