Difference between revisions of "Part:BBa K845008:Design"

(→‎Design Notes)
(→‎References)
 
(3 intermediate revisions by the same user not shown)
Line 7: Line 7:
 
===Design Notes===
 
===Design Notes===
  
−
This gene has to be inserted in <i>&Delta;cyaA-</i> bacteria.
+
This gene has to be inserted in <i>&Delta;cyaA</i> bacteria.
  
  
Line 14: Line 14:
 
===Source===
 
===Source===
  
−
...
+
Extracted from BW25113 <i>E. Coli</i> strain
  
 
===References===
 
===References===
Line 32: Line 32:
  
 
[8] Jan Weber†, Anke Kayser‡ and Ursula Rinas. Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. II. Dynamic response to famine and feast, activation of the methylglyoxal pathway and oscillatory behaviour. Microbiology March 2005 vol. 151 no. 3 707-716
 
[8] Jan Weber†, Anke Kayser‡ and Ursula Rinas. Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. II. Dynamic response to famine and feast, activation of the methylglyoxal pathway and oscillatory behaviour. Microbiology March 2005 vol. 151 no. 3 707-716
 +
 +
http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10170
 +
 +
http://www.uniprot.org/uniprot/P00936

Latest revision as of 13:41, 30 September 2012

cyaA with its natural RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 288
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 922
    Illegal SapI.rc site found at 412
    Illegal SapI.rc site found at 652


Design Notes

This gene has to be inserted in ΔcyaA bacteria.


Safety note: https://static.igem.org/mediawiki/2012/4/48/CyaA.pdf

Source

Extracted from BW25113 E. Coli strain

References

[1] Gorke, B., & Stulke, J. 2008. Carbon catabolite repression in bacteria: many ways to make the most out of nutrients. Nat Rev Microbiol, 6(8), 613–24.

[2] Keseler, I. M., Bonavides-Martinez, C., Collado-Vides, J., Gama-Castro, S., Gunsalus, R. P., Johnson, D. A., Krummenacker, M., Nolan, L. M., Paley, S., Paulsen, I. T., Peralta-Gil, M., Santos-Zavaleta, A., Shearer, A. G., & Karp, P. D. 2009. EcoCyc: a comprehensive view of Escherichia coli biology. Nucleic Acids Res, 37(Database issue), D464–70. [3] Goldenbaum, P. E., & Hall, G. A. 1979. Transport of cyclic adenosine 3’,5’-monophosphate across Escherichia coli vesicle membranes. J Bacteriol, 140(2), 459–67.

[4] Makman, R. S., & Sutherland, E. W. 1965. Adenosine 3’,5’-Phosphate in Escherichia Coli. J Biol Chem, 240, 1309–14.

[5] K Mori and H Aiba

[6] Cell. 1983 Jan;32(1):141-9. Autoregulation of the Escherichia coli crp gene: CRP is a transcriptional repressor for its own gene. Aiba H

[7] Rollie S. Ackerman, Nicholas R. Cozzarelli and Wolfgang Epstein.Accumulation of Toxic Concentrations of Methylglyoxal by Wild-Type Escherichia coli K-12.J. Bacteriol. August 1974 vol. 119 no. 2 357-362

[8] Jan Weber†, Anke Kayser‡ and Ursula Rinas. Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. II. Dynamic response to famine and feast, activation of the methylglyoxal pathway and oscillatory behaviour. Microbiology March 2005 vol. 151 no. 3 707-716

http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10170

http://www.uniprot.org/uniprot/P00936