Difference between revisions of "Part:BBa K771106"
(One intermediate revision by the same user not shown) | |||
Line 7: | Line 7: | ||
[[Image:12SJTU GBDdl.gif|right]] | [[Image:12SJTU GBDdl.gif|right]] | ||
− | The GBD ligand along with its domain([https://parts.igem.org/Part:BBa_K771105 Part:BBa_K771105]) comes from '' | + | The GBD ligand along with its domain([https://parts.igem.org/Part:BBa_K771105 Part:BBa_K771105]) comes from ''Rattus rattus'', which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the GBD ligand is located in Membrane Anchor 5, while the GBD domain on Membrane Anchor 4. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 4. |
===Related Parts=== | ===Related Parts=== | ||
− | |||
− | [https://parts.igem.org/Part: | + | [https://parts.igem.org/Part:BBa_K771106 Part:BBa_K771105, GBD domain] |
+ | |||
+ | |||
+ | [https://parts.igem.org/Part:BBa_K771004 Part:BBa_K771004, Membrane Anchor 4, containing GBD domain] | ||
+ | |||
+ | |||
+ | [https://parts.igem.org/Part:BBa_K771005 Part:BBa_K771005, Membrane Anchor 5, containing GBD ligand] | ||
+ | |||
− | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K771106 SequenceAndFeatures</partinfo> | <partinfo>BBa_K771106 SequenceAndFeatures</partinfo> |
Latest revision as of 03:56, 29 September 2012
GBDligand
components of interacting proteins
Usage and Biology
The GBD ligand along with its domain(Part:BBa_K771105) comes from Rattus rattus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the GBD ligand is located in Membrane Anchor 5, while the GBD domain on Membrane Anchor 4. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 4.
Related Parts
Part:BBa_K771004, Membrane Anchor 4, containing GBD domain
Part:BBa_K771005, Membrane Anchor 5, containing GBD ligand
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]