Difference between revisions of "Part:BBa K892402"
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<partinfo>BBa_K892402 short</partinfo> | <partinfo>BBa_K892402 short</partinfo> | ||
− | A | + | A mutated HB36.5 flu binder aimed to increase binding to H2, a subtype of hemagglutinin on the surface of the Influenza virus. This mutant has a point mutation at residue 317 from Phenylalanine to Serine |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This part was tested by the [http://2012.igem.org/Team:Washington 2012 University of Washington] iGEM team to bind to various subtypes of Hemagglutinin in Group 1.To test BBa _K892401, it was inserted into a protein expression vector, pETCON. HB36.5_F317H was then produced and transformed into yeast as described in the [http://2012.igem.org/Team:Washington/Protocols/Yeast 2012 Team's Yeast Transformation Protocol]. The binding protein was then tested for activity against H2. For a detailed description of the methodology, please see the [http://2012.igem.org/Team:Washington/Protocols/Display 2012 UW iGEM Yeast Surface Display protocol]. The resulting data is shown below. | ||
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+ | [[Image:UWashington FLU HB36 results graph.png|border|800px|center|thumb|HB36.5 and its variants tested against hemagglutinin subtype 2. HB36.5 is shown to have very little activity in binding H2.]] | ||
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Latest revision as of 16:25, 3 October 2012
HB36.5 F317S
A mutated HB36.5 flu binder aimed to increase binding to H2, a subtype of hemagglutinin on the surface of the Influenza virus. This mutant has a point mutation at residue 317 from Phenylalanine to Serine
Usage and Biology
This part was tested by the [http://2012.igem.org/Team:Washington 2012 University of Washington] iGEM team to bind to various subtypes of Hemagglutinin in Group 1.To test BBa _K892401, it was inserted into a protein expression vector, pETCON. HB36.5_F317H was then produced and transformed into yeast as described in the [http://2012.igem.org/Team:Washington/Protocols/Yeast 2012 Team's Yeast Transformation Protocol]. The binding protein was then tested for activity against H2. For a detailed description of the methodology, please see the [http://2012.igem.org/Team:Washington/Protocols/Display 2012 UW iGEM Yeast Surface Display protocol]. The resulting data is shown below.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 107
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 107
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 289
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 107
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 107
- 1000COMPATIBLE WITH RFC[1000]