Difference between revisions of "Part:BBa K771001"
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<partinfo>BBa_K771001 short</partinfo>
 
<partinfo>BBa_K771001 short</partinfo>
  
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Membrane anchor 1:RNA aptamer binding protein PP7 fused with membrane protein Lgt. And it is a part of membrane anchor rudder system.
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Membrane anchor 1:RNA aptamer binding protein PP7 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) fused with membrane protein Lgt([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771102 BBa_K771102]).
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==membrane anchor rudder system==
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[[Image:12SJTU-MA1-MA6.png]]
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==RNA Rudder==
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==Overview: Membrane Scaffold System==
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[[Image:12SJTU RNASIGNAL.jpg|500px]]
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[[Image:12SJTU-MA1-MA6.png|500px|center]]
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Demonstration of the whole ''Membrane Scaffold'' device. Membrane Anchor 2, 3, 4, 5 consitutively aggregate while Membrane Anchor 1 and 6 only aggregate with constitutive cluster (Membrane Anchor 2, 3, 4, 5) when certain signal is present.
  
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A: Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 protein to our membrane device, RNA molecule in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding proteins.
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==Backgroud==
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==Light Rudder==
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Related Biobrick:
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Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway （in this case involving enzyme 1, 2, 3, 4 and 5) is activated or not. RNA molecule D0([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771009 BBa_K771009]) with PP7 and MS2 aptamer domain acts as the signal.
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[[Image:12SJTU RNASIGNAL.png|200px|center]]
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<br/>A:Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 RNA biding domain ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111] [https://parts.igem.org/wiki/index.php?title=Part:BBa_K771112 BBa_K771112]) to our membrane device, RNA molecule D0([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771009 BBa_K771009]) in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding enzymes.
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==Design==
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[[Image:12SJTU_MA1.png|200px|center]]
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RNA aptamer binding protein PP7([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) fused with membrane protein Lgt ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771102 BBa_K771102]). When RNA molecule D0 is present, Membrane Anchor 1 is expected to dimerize with Membrane Anchor 2([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771002 BBa_K771002]) through MS2([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771112 BBa_K771112]) and PP7([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) domain.
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==Related Biobrick==
 
<br\>Membrabe Anchor 2([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771002 BBa_K771002]])
 
<br\>Membrabe Anchor 2([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771002 BBa_K771002]])
 
<br\>Membrabe Anchor 3([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771003 BBa_K771003]])
 
<br\>Membrabe Anchor 3([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771003 BBa_K771003]])
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<br\>Membrabe Anchor 5([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771005 BBa_K771005]])
 
<br\>Membrabe Anchor 5([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771005 BBa_K771005]])
 
<br\>Membrabe Anchor 6([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771006 BBa_K771006]])
 
<br\>Membrabe Anchor 6([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771006 BBa_K771006]])
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==Reference==
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Delebecque, C. J., A. B. Lindner, et al. (2011). "Organization of intracellular reactions with rationally designed RNA assemblies." Science 333(6041): 470.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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===Usage and Biology===
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==Usage and Biology==
  
 
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Latest revision as of 01:12, 30 September 2012

Membrane Anchor 1 :ssDsbA-Lgt-PP7

Membrane anchor 1:RNA aptamer binding protein PP7 (BBa_K771111) fused with membrane protein Lgt(BBa_K771102).

Overview: Membrane Scaffold System

12SJTU-MA1-MA6.png

Demonstration of the whole Membrane Scaffold device. Membrane Anchor 2, 3, 4, 5 consitutively aggregate while Membrane Anchor 1 and 6 only aggregate with constitutive cluster (Membrane Anchor 2, 3, 4, 5) when certain signal is present.

Backgroud

Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway （in this case involving enzyme 1, 2, 3, 4 and 5) is activated or not. RNA molecule D0(BBa_K771009) with PP7 and MS2 aptamer domain acts as the signal.

12SJTU RNASIGNAL.png


A:Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 RNA biding domain (BBa_K771111 BBa_K771112) to our membrane device, RNA molecule D0(BBa_K771009) in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding enzymes.

Design

12SJTU MA1.png

RNA aptamer binding protein PP7(BBa_K771111) fused with membrane protein Lgt (BBa_K771102). When RNA molecule D0 is present, Membrane Anchor 1 is expected to dimerize with Membrane Anchor 2(BBa_K771002) through MS2(BBa_K771112) and PP7(BBa_K771111) domain.

Related Biobrick

<br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])


Reference

Delebecque, C. J., A. B. Lindner, et al. (2011). "Organization of intracellular reactions with rationally designed RNA assemblies." Science 333(6041): 470.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1129
    Illegal BglII site found at 1385
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 504