Difference between revisions of "Part:BBa K771001"
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<partinfo>BBa_K771001 short</partinfo>
 
<partinfo>BBa_K771001 short</partinfo>
  
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RNA aptamer binding protein PP7 fused with membrane protein Lgt
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Membrane anchor 1:RNA aptamer binding protein PP7 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) fused with membrane protein Lgt([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771102 BBa_K771102]).
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==Overview: Membrane Scaffold System==
 
[[Image:12SJTU-MA1-MA6.png|500px|center]]
 
[[Image:12SJTU-MA1-MA6.png|500px|center]]
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Demonstration of the whole ''Membrane Scaffold'' device. Membrane Anchor 2, 3, 4, 5 consitutively aggregate while Membrane Anchor 1 and 6 only aggregate with constitutive cluster (Membrane Anchor 2, 3, 4, 5) when certain signal is present.
  
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==Backgroud==
  
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As there is no compartment in prokaryotic cells, enzymes involved in a biochemical pathway diffuse all over the cytoplasm. Intermediates produced from one enzyme cannot be passed efficiently to the next due to spatial obstacles.
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Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway （in this case involving enzyme 1, 2, 3, 4 and 5) is activated or not. RNA molecule D0([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771009 BBa_K771009]) with PP7 and MS2 aptamer domain acts as the signal.
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Rationally designed assemblies of enzymes could help substrates flow by decreasing distance that intermediates have to travel between enzymes, and thus increase yields of sequential biological reactions. If we attach those enzymes to engineered membrane proteins which constitutively assemble together, the reactions are going to proceed much faster.
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Related Biobrick:
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[[Image:12SJTU RNASIGNAL.png|200px|center]]
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<br/>A:Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 RNA biding domain ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111] [https://parts.igem.org/wiki/index.php?title=Part:BBa_K771112 BBa_K771112]) to our membrane device, RNA molecule D0([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771009 BBa_K771009]) in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding enzymes.
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==Design==
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[[Image:12SJTU_MA1.png|200px|center]]
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RNA aptamer binding protein PP7([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) fused with membrane protein Lgt ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771102 BBa_K771102]). When RNA molecule D0 is present, Membrane Anchor 1 is expected to dimerize with Membrane Anchor 2([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771002 BBa_K771002]) through MS2([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771112 BBa_K771112]) and PP7([https://parts.igem.org/wiki/index.php?title=Part:BBa_K771111 BBa_K771111]) domain.
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==Related Biobrick==
 
<br\>Membrabe Anchor 2([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771002 BBa_K771002]])
 
<br\>Membrabe Anchor 2([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771002 BBa_K771002]])
 
<br\>Membrabe Anchor 3([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771003 BBa_K771003]])
 
<br\>Membrabe Anchor 3([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771003 BBa_K771003]])
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<br\>Membrabe Anchor 5([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771005 BBa_K771005]])
 
<br\>Membrabe Anchor 5([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771005 BBa_K771005]])
 
<br\>Membrabe Anchor 6([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771006 BBa_K771006]])
 
<br\>Membrabe Anchor 6([[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771006 BBa_K771006]])
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==Reference==
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Delebecque, C. J., A. B. Lindner, et al. (2011). "Organization of intracellular reactions with rationally designed RNA assemblies." Science 333(6041): 470.
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===Usage and Biology===
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==Usage and Biology==
  
 
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Latest revision as of 01:12, 30 September 2012

Membrane Anchor 1 :ssDsbA-Lgt-PP7

Membrane anchor 1:RNA aptamer binding protein PP7 (BBa_K771111) fused with membrane protein Lgt(BBa_K771102).

Overview: Membrane Scaffold System

12SJTU-MA1-MA6.png

Demonstration of the whole Membrane Scaffold device. Membrane Anchor 2, 3, 4, 5 consitutively aggregate while Membrane Anchor 1 and 6 only aggregate with constitutive cluster (Membrane Anchor 2, 3, 4, 5) when certain signal is present.

Backgroud

Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway （in this case involving enzyme 1, 2, 3, 4 and 5) is activated or not. RNA molecule D0(BBa_K771009) with PP7 and MS2 aptamer domain acts as the signal.

12SJTU RNASIGNAL.png


A:Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 RNA biding domain (BBa_K771111 BBa_K771112) to our membrane device, RNA molecule D0(BBa_K771009) in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding enzymes.

Design

12SJTU MA1.png

RNA aptamer binding protein PP7(BBa_K771111) fused with membrane protein Lgt (BBa_K771102). When RNA molecule D0 is present, Membrane Anchor 1 is expected to dimerize with Membrane Anchor 2(BBa_K771002) through MS2(BBa_K771112) and PP7(BBa_K771111) domain.

Related Biobrick

<br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])


Reference

Delebecque, C. J., A. B. Lindner, et al. (2011). "Organization of intracellular reactions with rationally designed RNA assemblies." Science 333(6041): 470.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1129
    Illegal BglII site found at 1385
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 504