Difference between revisions of "Part:BBa K778002"

Latest revision as of 05:49, 3 October 2012

fhlA-E363G (engineered trascriptional activator fhlA with a mutation)

FhlA is the transcriptional activator of hycAp Part:BBa K778000 .E.coli overexpressing FhlA produce H₂ (since hycAp is the promoter of FHL(formate hydrogen lyase)).

fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from wild-type fhlA. This mutation increased H₂ production under the conditions in the previous research (Viviana et.al.2009). Actually, in our research (team UT-Tokyo 2012), this mutation increased H₂ production as the following figure. (The full construct in this experiment is Part:BBa K778008)

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] but the results in this wiki is older than this figure above.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2096
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1375
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K778002 short</partinfo>
-FhlA is the trnascriptional activator of hycAp [[Part:BBa K778000]] .''E.coli'' overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
+FhlA is the transcriptional activator of hycAp [[Part:BBa K778000]] .''E.coli'' overexpressing FhlA produce H<sub>2</sub> (since hycAp is the promoter of FHL(formate hydrogen lyase)).
-fhlA-E363G has one point mutation (A to G, glutamic acid to glycine).
+fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from [[Part:BBa K778001|wild-type fhlA]].
-This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009).
+This mutation increased H<sub>2</sub>  production under the conditions in the previous research (Viviana et.al.2009).
-However, in our  research (team UT-Tokyo 2012[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]), this mutation did not increase H2 production.
+Actually, in our research (team UT-Tokyo 2012<!--[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]-->), this mutation increased H<sub>2</sub> production as the following figure.<!--this mutation did not increase H2 production.--> (The full construct in this experiment is [[Part:BBa K778008]])
- Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]
+[[Image:UT Tokyo H2 1001+eg.png]]
+<!--[[Image:UT Tokyo H2 1001.png]]-->
+Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] but the results in this wiki is older than this figure above.
 <!-- Add more about the biology of this part here