Difference between revisions of "Part:BBa K540000:Experience"

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UCL iGEM 2012 redesigned this part with the purpose of increasing the adhesive properties of E. coli and marine bacteria Oceanibulbus indoliflex and Roseobacter denitrificans. To do so we removed the cobalt inducible promoter to produce a stand alone operon with rbs.
 
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We further modified this part by the addition of a constitutive promoter and T7 promoter, and verified curli expression using congo red binding assays.
 
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Stand alone part: BBa_K729003
 
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T7 promoter: BBa_K729017
 
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Constitutive promoter: BBa_K729018
 
  
 
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<I>UCL iGEM 2012</I>
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UCL iGEM 2012 have removed the original cobalt inducible promoter to create a stand alone csgBAEFG operon with rbs [https://parts.igem.org/wiki/index.php?title=Part:BBa_K729003 BBa_K729003]. We have also designed two additional variations on the part, with a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K729017 T7] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K729018 constitutive promoter].
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Latest revision as of 19:30, 28 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K540000

User Reviews

UNIQfc551152ad4c2653-partinfo-00000000-QINU UNIQfc551152ad4c2653-partinfo-00000001-QINU

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UCL iGEM 2012

UCL iGEM 2012 have removed the original cobalt inducible promoter to create a stand alone csgBAEFG operon with rbs BBa_K729003. We have also designed two additional variations on the part, with a T7 and constitutive promoter.