Difference between revisions of "Part:BBa K861013:Experience"

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<I>iGEM2012 WHU-China</I>
 
<I>iGEM2012 WHU-China</I>
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<p>In the in vitro assay we performed, we collected cell extracts from 1L medium and got protein solution of high concentration .The enzyme assay was then performed to measure the ability of degrading fatty acids by single enzyme and their different combinations.The remaining amount of fatty acids in the medium is measured by the cupric-acetate reaction .
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<p>In the in vitro assay we performed, we collected cell extracts from 1L medium and got protein solution of high concentration. The enzyme assay was then performed to measure the ability of degrading fatty acids by single enzyme and their different combinations. The remaining amount of fatty acids in the medium was measured by the cupric-acetate reaction.
  
 
https://static.igem.org/mediawiki/parts/b/bc/Cupric_acetate-1-.png
 
https://static.igem.org/mediawiki/parts/b/bc/Cupric_acetate-1-.png
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https://static.igem.org/mediawiki/parts/d/d9/%E4%BD%93%E5%A4%96%E5%AE%9E%E9%AA%8C.jpg<br/>
  
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In this picture , the color of the sample     standing for cell extracts collected from bacteria overexpressing fadD is light than the controls . It means the fatty acids remaining in the tube is less .From the results above, we have proved that the biobrick responsible for overexpressing fadD can metabolize fatty acids much faster.
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 +
In this picture, the color of the sample E standing for cell extracts collected from bacteria overexpressing fadD was lighter than the control(tube A). It means the fatty acids remaining in the tube was less. From the results above, we have proved that the biobrick responsible for overexpressing fadD can metabolize fatty acids much faster.
  
 
For more details, please visit our wiki http://2012.igem.org/Team:WHU-China
 
For more details, please visit our wiki http://2012.igem.org/Team:WHU-China

Latest revision as of 16:56, 29 September 2012

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Applications of BBa_K861013

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iGEM2012 WHU-China

In the in vitro assay we performed, we collected cell extracts from 1L medium and got protein solution of high concentration. The enzyme assay was then performed to measure the ability of degrading fatty acids by single enzyme and their different combinations. The remaining amount of fatty acids in the medium was measured by the cupric-acetate reaction. Cupric_acetate-1-.png %E4%BD%93%E5%A4%96%E5%AE%9E%E9%AA%8C.jpg
In this picture, the color of the sample E standing for cell extracts collected from bacteria overexpressing fadD was lighter than the control(tube A). It means the fatty acids remaining in the tube was less. From the results above, we have proved that the biobrick responsible for overexpressing fadD can metabolize fatty acids much faster. For more details, please visit our wiki http://2012.igem.org/Team:WHU-China

;

User Reviews

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syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_"
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Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 250.
Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line
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Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288.
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