Difference between revisions of "Part:BBa K945000"

Latest revision as of 10:47, 6 October 2012

AOX1 yeast promoter

This sequence contains the strong inducible transcription promoter AOX1, useful for controlled gene expression which is actively enhanced in Pichia pastoris in the presence of methanol.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1016
Illegal PstI site found at 47
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 47
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 53
Illegal BglII site found at 75
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1016
Illegal PstI site found at 47
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1016
Illegal PstI site found at 47
Illegal NgoMIV site found at 34
1000
COMPATIBLE WITH RFC[1000]

Promoter Strength

Tec-Monterrey Ekam is working on quantitatively characterizing the functionality of this part through the use of BBa_K945004 (GFP) as a reporter, which will be finalized shortly.

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K945000 short</partinfo>
-This sequence contains the strong inducible transcription promoter AOX1, useful for controlled gene expression which is actively enhanced in 'Pichia pastoris' in the presence of methanol.
+This sequence contains the strong inducible transcription promoter AOX1, useful for controlled gene expression which is actively enhanced in <i>Pichia pastoris</i> in the presence of methanol.
 <!-- Add more about the biology of this part here
@@ Line 11: / Line 11: @@
 <partinfo>BBa_K945000 SequenceAndFeatures</partinfo>
+===Promoter Strength===
+Tec-Monterrey Ekam is working on '''quantitatively''' characterizing the functionality of this part through the use of <partinfo>BBa_K945004</partinfo> (GFP) as a reporter, which will be finalized shortly.
 <!-- Uncomment this to enable Functional Parameter display