Difference between revisions of "Part:BBa K899004"
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| − | <span class='h3bb'>This construct was designed with the BBa_R0010 in order to control transcription. | + | <span class='h3bb'>This construct was designed with the promoter BBa_R0010 in order to control transcription. We suspect transcription of 1-SST and 1-FFT to greatly inhibit bacterial growth with the production of plant inulin. LacI protein and CAP can succesfully inhibit transcription of both genes to allow the bacteria to grow. With the addition of IPTG to the medium, LacI will be inhibited and transcription can resume.</span> |
<partinfo>BBa_K899004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K899004 SequenceAndFeatures</partinfo> | ||
Latest revision as of 02:07, 27 September 2012
Reporter system for SST and FFT (BBa_K899000 and BBa_K899001)
Reporter system to test for transcription of fructan:fructan fructosyltransferase (FFT) and Sucrose:sucrose fructosyltransferase (SST) genes with green flourescent protein (GFP) as reporter, and lacl as promoter.
This construct was designed with the promoter BBa_R0010 in order to control transcription. We suspect transcription of 1-SST and 1-FFT to greatly inhibit bacterial growth with the production of plant inulin. LacI protein and CAP can succesfully inhibit transcription of both genes to allow the bacteria to grow. With the addition of IPTG to the medium, LacI will be inhibited and transcription can resume.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2053
Illegal BglII site found at 2662
Illegal BamHI site found at 346
Illegal BamHI site found at 836 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 297
Illegal AgeI site found at 2516 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4666