Difference between revisions of "Part:BBa K953000"
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| − | <i>Synechocystis</i> Heme Oxygenase with a RBS and codon optimised for expression in <i>E. coli</i>. This part differs from <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953004">Part BBa_K953004</a></html> in that it also includes a T7 promoter (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">Part BBa_I712074</a></html>). Heme oxygenase will oxidise heme into biliverdin and can then complex with bacteriophytochrome(<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953001">Part BBa_K953001</a></html> or <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953002">Part BBa_K953002</a></html>). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase | + | <i>Synechocystis</i> Heme Oxygenase with a RBS and codon optimised for expression in <i>E. coli</i>. This part differs from <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953004">Part BBa_K953004</a></html> in that it also includes a T7 promoter (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">Part BBa_I712074</a></html>). Heme oxygenase will oxidise heme into biliverdin and can then complex with bacteriophytochrome (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953001">Part BBa_K953001</a></html> or <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K953002">Part BBa_K953002</a></html>). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As <i>E. coli</i> does not produce biliverdin, heme oxygenase must be coupled with a bacteriophytochrome to activate the oxidation of heme to produce biliverdin. |
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| + | The results and characterisation of heme oxygenase can be found <html><a href="http://2012.igem.org/Team:Macquarie_Australia/Results#1">here</a></html> and you can also check out our entire project be clicking <html><a href="http://2012.igem.org/Team:Macquarie_Australia">here.</a></html> | ||
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| + | <center>http://25.media.tumblr.com/tumblr_mazgigGnrs1rg4kjpo1_500.jpg</center> | ||
| + | <center><i>Pellets of cells containing induced heme oxygenase, uninducded heme oxygenase and lacking heme oxygenase</i></center> | ||
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| + | <br> | ||
Latest revision as of 02:21, 27 September 2012
Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)
Synechocystis Heme Oxygenase with a RBS and codon optimised for expression in E. coli. This part differs from Part BBa_K953004 in that it also includes a T7 promoter (Part BBa_I712074). Heme oxygenase will oxidise heme into biliverdin and can then complex with bacteriophytochrome (Part BBa_K953001 or Part BBa_K953002). This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase must be coupled with a bacteriophytochrome to activate the oxidation of heme to produce biliverdin.
The results and characterisation of heme oxygenase can be found here and you can also check out our entire project be clicking here.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 314
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]