Difference between revisions of "Part:BBa K743015:Experience"
 
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'''[http://2012.igem.org/Team:UC_Chile Team UC_Chile]'''
 
'''[http://2012.igem.org/Team:UC_Chile Team UC_Chile]'''
 
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We placed two versions of the LuxAB genes under this promoter to test its expression in Synechocystis PCC6803 depending on the addition of two aldehydes substrates: decanal and dodecanal.
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We tested this device in Synechocystis PCC6803 depending on the addition of two aldehydes substrates: decanal and dodecanal.
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Expression was measured on a Termo Scientific Luminoskan Ascent luminometer for microplates, for more details on the experimental design go to Promoters Luciferase Assay at our [http://2012.igem.org/Team:UC_Chile/Protocols protocols page].
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Expression was measured on a Termo Scientific Luminoskan Ascent luminometer for microplates. For more details on the experimental design go to Promoters Luciferase Assay at our [http://2012.igem.org/Team:UC_Chile/Protocols protocols page].
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[[Image:potopotopoto.jpg]]
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[[Image:cacacacax56t.jpg]]
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[[Image:potopotopoto.jpg|500px]]
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Upon addition, decanal is used as a substrate for the LuxAB luciferase reaction in E.coli cells transformed with the LuxBrick. Synechocystis cells transformed with K743015 don´t show luminescence, indicating that probably the Pta promoter is not being recognized by the transcriptional machinery.
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[[Image:cacacacax56t.jpg|500px]]
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Upon addition, dodecanal is used as a substrate for the LuxAB luciferase reaction in E.coli cells transformed with the LuxBrick. Synechocystis cells transformed with K743015 don´t show luminescence, indicating that probably the Pta promoter is not being recognized by the transcriptional machinery.
 
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Latest revision as of 00:54, 27 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K743015

User Reviews

[http://2012.igem.org/Team:UC_Chile Team UC_Chile]
We tested this device in Synechocystis PCC6803 depending on the addition of two aldehydes substrates: decanal and dodecanal. Expression was measured on a Termo Scientific Luminoskan Ascent luminometer for microplates. For more details on the experimental design go to Promoters Luciferase Assay at our [http://2012.igem.org/Team:UC_Chile/Protocols protocols page].
Potopotopoto.jpg
Upon addition, decanal is used as a substrate for the LuxAB luciferase reaction in E.coli cells transformed with the LuxBrick. Synechocystis cells transformed with K743015 don´t show luminescence, indicating that probably the Pta promoter is not being recognized by the transcriptional machinery.
Cacacacax56t.jpg
Upon addition, dodecanal is used as a substrate for the LuxAB luciferase reaction in E.coli cells transformed with the LuxBrick. Synechocystis cells transformed with K743015 don´t show luminescence, indicating that probably the Pta promoter is not being recognized by the transcriptional machinery.

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