Difference between revisions of "Part:BBa K812030"
 
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</br>This part was characterized in pCS2+ <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a> which contains the CMV promoter. </br> J04450 was replaced by this part, it built the BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812130">BBa_K812130</a>. The plasmid (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812130">BBa_K812130</a>) which contains this part express mCitrine into <i>Xenopus</i>: <br/>
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</br>This part was characterized in the biobrick version of  pCS2+ (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>) which contains the CMV promoter </br>  
  
 
Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles.
 
Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles.
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<img src="https://static.igem.org/mediawiki/2012/b/b6/Picture_citrine_7.09.jpg" alt="perdu" width="500px" /><br/>
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<img src="https://static.igem.org/mediawiki/2012/b/b6/Picture_citrine_7.09.jpg" alt="perdu" width="700px" /><br/>
  
 
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Latest revision as of 01:07, 27 October 2012

mCitrine reporter with a Kozak sequence for eukaryotic expression

mCitrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This part is in pSB1C3.


This part was characterized in the biobrick version of pCS2+ (BBa_K812000) which contains the CMV promoter
Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles.
perdu

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 667
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]