Difference between revisions of "Part:BBa K812132"

Latest revision as of 16:56, 30 September 2012

mCFP with kozak sequence for expression in Xenopus

*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812032. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000.***

mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (K812000) and related parts.

<img src="" alt="perdu" width="880px" />
</html> Tadpole 1 is 2 days (stage 36-38), the expression of CFP is localized in intestine. Tadpole 2 is 4 days (stage 45-46), the expression of CFP is localized in Branchial basket and intestine

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K812132 short</partinfo>
-mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ <html>(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">K812000</a>)</html> and related parts.
 <html>
+</br>
+<b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812032">BBa_K812032</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>.***</b>
+</br></br>
+mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ <html>(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">K812000</a>)</html> and related parts.
 <br>
 <img src="https://static.igem.org/mediawiki/2012/5/58/CFP_tadpole.jpg" alt="perdu" width="880px" />