Difference between revisions of "Part:BBa K775005"
 
(3 intermediate revisions by the same user not shown)
Line 10: Line 10:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K775005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K775005 SequenceAndFeatures</partinfo>
−
<br>
+
 
−
[[Image:FUSGFP_Growth_Intensity_Div.png|960px]]
+
−
<br>
+
−
DNA intensity of stained S. cerevisiae cells at t=5.10. Please also see our page: [http://2012.igem.org/Team:TU-Delft/part2 Reporter]
+
−
<br>
+
−
[[Image:FUSGFP_Div_Time.png|960px]]
+
  
  
Line 22: Line 17:
 
<partinfo>BBa_K775005 parameters</partinfo>
 
<partinfo>BBa_K775005 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
<html>
 +
<body>
 +
<img src="https://static.igem.org/mediawiki/igem.org/b/b3/Screen-capture-14.jpg" alt="Niacin microscopy data"/>
 +
<p>Microscopy data, intensity based on maximum cell fluorescence. Pixel saturation is 250 (A.U.). Measurement based on ~400 cells, pictures are taken on random spots the culture plate.</p>
 +
</body>
 +
</html>

Latest revision as of 20:38, 29 October 2012

Niacin Chimeric GPCR with Fus1-EGFP reporter

This part is a combination between Bba_K775000(Niacin chimeric GPCR)and Bba_K775004 (Fus1-EGFP repoter).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 582
    Illegal BamHI site found at 113
    Illegal BamHI site found at 1623
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 478



Niacin microscopy data

Microscopy data, intensity based on maximum cell fluorescence. Pixel saturation is 250 (A.U.). Measurement based on ~400 cells, pictures are taken on random spots the culture plate.