Difference between revisions of "Part:BBa K797013"
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Golden gate assembly is a suitable assembly to combine many parts in order you want. | Golden gate assembly is a suitable assembly to combine many parts in order you want. | ||
This plasmid backbone have restriction enzyme cutting site of Dpn1 let you build construction by Golden gate assembly in one ligation. | This plasmid backbone have restriction enzyme cutting site of Dpn1 let you build construction by Golden gate assembly in one ligation. | ||
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We conducted Golden gate assembly by ourselves and confirmed this part has the restriction cite and restriction enzyme cutting site of Bsa1. After this, we mixed Dpn1 with enzyme-treated plasmid and conducted ligation. | We conducted Golden gate assembly by ourselves and confirmed this part has the restriction cite and restriction enzyme cutting site of Bsa1. After this, we mixed Dpn1 with enzyme-treated plasmid and conducted ligation. |
Latest revision as of 23:14, 26 September 2012
Plasmid Backbone for golden gate assembly
This backbone is suitable for golden gate assembly. Golden gate assembly is a suitable assembly to combine many parts in order you want. This plasmid backbone have restriction enzyme cutting site of Dpn1 let you build construction by Golden gate assembly in one ligation.
We conducted Golden gate assembly by ourselves and confirmed this part has the restriction cite and restriction enzyme cutting site of Bsa1. After this, we mixed Dpn1 with enzyme-treated plasmid and conducted ligation.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 2197
Illegal suffix found at 15 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2197
Illegal SpeI site found at 16
Illegal PstI site found at 30
Illegal NotI site found at 23
Illegal NotI site found at 2203 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2197
Illegal XhoI site found at 1047
Illegal XhoI site found at 2073 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2197
Illegal suffix found at 16 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2197
Plasmid lacks a suffix.
Illegal XbaI site found at 2212
Illegal SpeI site found at 16
Illegal PstI site found at 30 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 4
Illegal BsaI.rc site found at 2224