Difference between revisions of "Part:BBa K812010"
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<partinfo>BBa_K812010 short</partinfo> | <partinfo>BBa_K812010 short</partinfo> | ||
| − | This part is a | + | This part is a GFP coding sequence fused to an AID tag for its recognition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. This is designed to be used in pSB1C3 vector. |
This part is a part of a device patented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and | This part is a part of a device patented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and | ||
| − | Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the | + | Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the patent does not cover the use for oviparian such as frogs and chicken. |
The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration. | The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration. | ||
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This device was caracterised in pCS2+ plasmid <html>(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>)</html>. | This device was caracterised in pCS2+ plasmid <html>(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>)</html>. | ||
<html><br> | <html><br> | ||
| + | <br> | ||
<img src="https://static.igem.org/mediawiki/parts/c/ce/Picture_genethon_GFP-aid_7.09.jpg" alt="perdu" width="880px" /> <br/><br/> | <img src="https://static.igem.org/mediawiki/parts/c/ce/Picture_genethon_GFP-aid_7.09.jpg" alt="perdu" width="880px" /> <br/><br/> | ||
| − | |||
The tadpole 1 express GFP-aid in epidermic cells, whereas the tadpole 2 express GFP-aid in optical nerve,nostril nerve, tail muscle cells and branchial basket. | The tadpole 1 express GFP-aid in epidermic cells, whereas the tadpole 2 express GFP-aid in optical nerve,nostril nerve, tail muscle cells and branchial basket. | ||
| + | <br> | ||
| + | <br> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/5/5a/Degron1.jpg" alt="perdu" width="1000px" /> <br/><br/> | ||
| + | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 00:02, 27 October 2012
GFP fused with AID-tag (ubuquitinase E3 OsTirI recoginition domain)
This part is a GFP coding sequence fused to an AID tag for its recognition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. This is designed to be used in pSB1C3 vector.
This part is a part of a device patented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the patent does not cover the use for oviparian such as frogs and chicken.
The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration.
This device was caracterised in pCS2+ plasmid (BBa_K812000).
The tadpole 1 express GFP-aid in epidermic cells, whereas the tadpole 2 express GFP-aid in optical nerve,nostril nerve, tail muscle cells and branchial basket.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1020
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1405