Difference between revisions of "Part:BBa K540000:Experience"

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UCL iGEM 2012 modified BBa_K540000 for the purpose of increasing the adhesive properties of E. coli and marine bacteria of the roseobacter clade.
 
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As such we have removed the original cobalt induced promoter, to produce a stand alone csgBAEFG operon with rbs [BBa_K729003[https://parts.igem.org/wiki/index.php?title=Part:BBa_K729003]].
 
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We have also designed two additional constructs with a constitutive and T7 promoter and demonstrated successful expression of the csgBAEFG operon through congo red binding assays, BBa_K729017 and BBa_K729018.
 
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<I>UCL iGEM 2012</I>
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UCL iGEM 2012 have removed the original cobalt inducible promoter to create a stand alone csgBAEFG operon with rbs [https://parts.igem.org/wiki/index.php?title=Part:BBa_K729003 BBa_K729003]. We have also designed two additional variations on the part, with a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K729017 T7] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K729018 constitutive promoter].
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Latest revision as of 19:30, 28 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K540000

User Reviews

UNIQ7b1449013e539007-partinfo-00000000-QINU UNIQ7b1449013e539007-partinfo-00000001-QINU

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UCL iGEM 2012

UCL iGEM 2012 have removed the original cobalt inducible promoter to create a stand alone csgBAEFG operon with rbs BBa_K729003. We have also designed two additional variations on the part, with a T7 and constitutive promoter.