Difference between revisions of "Part:BBa K801011:Design"

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'''Cloning details:'''<br> Designed in RFC10/RFC23/RFC25/RFC21
 
'''Cloning details:'''<br> Designed in RFC10/RFC23/RFC25/RFC21
 +
* Mutation in NotI in Suffix
 
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
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===Source===
 
===Source===
  
−
'''Source:'''<br>
+
'''Source:'''<br> PCR product from genomic DNA of ''Saccharomyces cerevisiae''
 
<!--*Commercial system: plasmid name, system name, company name-->
 
<!--*Commercial system: plasmid name, system name, company name-->
 
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
 
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
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<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 
<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
  
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'''Organism:'''<br>
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'''Organism:'''<br> ''Saccharomyces cerevisiae''
 
<!--*Genesequence derived from ''?organism_name?''-->
 
<!--*Genesequence derived from ''?organism_name?''-->
 
<!--*Codonoptimized for ''?organism_name?''-->
 
<!--*Codonoptimized for ''?organism_name?''-->
 
<!--*Designed for the following Chassis: ''?organism-name?''-->
 
<!--*Designed for the following Chassis: ''?organism-name?''-->
 
<!--*Statement about functionality in other chassis.-->
 
<!--*Statement about functionality in other chassis.-->
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===References===
 
===References===
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'''Literature references:'''<br>
 
'''Literature references:'''<br>
 +
*Sun, Jie; Shao, Zengyi; Zhao, Hua; Nair, Nikhil; Wen, Fei; Xu, Jian-He; Zhao, Huimin (2012): Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. In: Biotechnol. Bioeng. 109 (8), S. 2082–2092.
 +
*Guo, Z.; Sherman, F. (1996): 3'-end-forming signals of yeast mRNA. In: Trends Biochem. Sci. 21 (12), S. 477–481.
 +
 
<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
 
<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
  
 
'''Database references:'''<br>
 
'''Database references:'''<br>
 +
 
<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
 
<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
 
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
 
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->

Latest revision as of 22:45, 26 September 2012

TEF1 yeast terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.



Extracted from genomic DNA of S. cerevisiae using PCR.


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:
Test digestion using XbaI+PstI-HF, EcorI+ SpeI-HF

  • Sequencing using primer VR and VF2

Backbone:
Backbone name: pSB1C3

Protein coding:

Enzymatic activity:

Cytotoxicity:
none

Safety notes:
none

Intellectual property:

Corresponding part author/authors:

Source

Source:
PCR product from genomic DNA of Saccharomyces cerevisiae


Organism:
Saccharomyces cerevisiae

References

Literature references:

  • Sun, Jie; Shao, Zengyi; Zhao, Hua; Nair, Nikhil; Wen, Fei; Xu, Jian-He; Zhao, Huimin (2012): Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. In: Biotechnol. Bioeng. 109 (8), S. 2082–2092.
  • Guo, Z.; Sherman, F. (1996): 3'-end-forming signals of yeast mRNA. In: Trends Biochem. Sci. 21 (12), S. 477–481.


Database references: