Difference between revisions of "Part:BBa K778010"
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K778010 short</partinfo>
 
<partinfo>BBa_K778010 short</partinfo>
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You can "enhance" and "see" H2 production with this device.
  
 
hycAp-GFPgenerator-plac-FhlAgenerator
 
hycAp-GFPgenerator-plac-FhlAgenerator
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FhlA is the trnascriptional activator of hycAp [[Part:BBa K778000]] .
 
FhlA is the trnascriptional activator of hycAp [[Part:BBa K778000]] .
 
E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
 
E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
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fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from [[Part:BBa K778001|wild-type fhlA]].
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This mutation increased H<sub>2</sub>  production under the conditions in the previous research (Viviana et.al.2009).
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Actually, in our research (team UT-Tokyo 2012<!--[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]-->), this mutation increased H<sub>2</sub> production as the following figure.<!--this mutation did not increase H2 production.--> (The full construct in this experiment is [[Part:BBa K778008]])
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[[Image:UT Tokyo H2 1001+eg.png]]
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<!--[[Image:UT Tokyo H2 1001.png]]-->
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Detailed Information :Our wiki (UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]
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The results shown on the wiki are different from the latest result on this parts.igem page.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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<partinfo>BBa_K778010 parameters</partinfo>
 
<partinfo>BBa_K778010 parameters</partinfo>
 
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[[Image:Safety.png]]

Latest revision as of 06:36, 6 October 2012

hycAp-GFPgenerator-plac-FhlAgenerator

You can "enhance" and "see" H2 production with this device.

hycAp-GFPgenerator-plac-FhlAgenerator

GFP is a reportor of H2 production (strictly speaking, reporter of hycAp activation Part:BBa K778000 (hycAp is the promotor of formate hydrogen lyase of genome)).

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from wild-type fhlA. This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009). Actually, in our research (team UT-Tokyo 2012), this mutation increased H2 production as the following figure. (The full construct in this experiment is Part:BBa K778008)

UT Tokyo H2 1001+eg.png

Detailed Information :Our wiki (UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] The results shown on the wiki are different from the latest result on this parts.igem page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2545
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 828


Safety.png