Difference between revisions of "Part:BBa K863102:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The Biobrick was designed to express the cellulose binding domain of ''Cellulomonas fimi'' exoglucanase (CBDcex) under the control of a T7-promoter. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and also a short C-terminal (Glycine, Serine) linker was added. The sequence ends with the ''Age''I-restriction-site of the Freiburg-Suffix, to make in frame [https://parts.igem.org/Assembly_standard_25 Standard 25 | + | The Biobrick was designed to express the cellulose binding domain of ''Cellulomonas fimi'' exoglucanase (CBDcex) under the control of a T7-promoter <partinfo>BBa_K525998</partinfo>. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and also a short C-terminal (Glycine, Serine) linker was added. The sequence ends with the ''Age''I-restriction-site of the Freiburg-Suffix, to make in-frame ([https://parts.igem.org/Assembly_standard_25 Standard 25]) assembly possible. |
+ | |||
+ | Primers that were used to make this BioBrick: | ||
+ | {| | ||
+ | |CBDcex_T7RBS||TGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAATAATGGGTCCGGCCGGGTGCCAGGT | ||
+ | |- | ||
+ | |CBDcex_2AS-Link_Frei||CTGCAGCGGCCGCTACTAGTATTAACCGGTGCTGCCGCCGACCGTGCAGGGCGTGC | ||
+ | |} | ||
===Source=== | ===Source=== |
Latest revision as of 20:44, 26 September 2012
Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 365
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Biobrick was designed to express the cellulose binding domain of Cellulomonas fimi exoglucanase (CBDcex) under the control of a T7-promoter BBa_K525998. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and also a short C-terminal (Glycine, Serine) linker was added. The sequence ends with the AgeI-restriction-site of the Freiburg-Suffix, to make in-frame (Standard 25) assembly possible.
Primers that were used to make this BioBrick:
CBDcex_T7RBS | TGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAATAATGGGTCCGGCCGGGTGCCAGGT |
CBDcex_2AS-Link_Frei | CTGCAGCGGCCGCTACTAGTATTAACCGGTGCTGCCGCCGACCGTGCAGGGCGTGC |
Source
Origin of the cellulose binding domain is a cloning-vector form the fermentation group of Bielefeld University.