Difference between revisions of "Part:BBa K778001:Design"

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===Design Notes===
 
===Design Notes===
−
Original fhlA gene we cloned from ''E. coli ''JM109 has recognition sites of PatⅠ.This illegal (inappropriate for biobrick) site was deleted by overlap extension PCR generating mutation (G to A).
+
Original fhlA gene we cloned from ''E. coli ''JM109 has recognition site of PstⅠ.This illegal (inappropriate for biobrick) site was deleted by overlap extension PCR generating mutation (G to A).
  
  
−
At 1906bp mutation (A to G, Thr to Ala) was found in this biobrick part(different from E. coli K-12 MG1655). can be
+
Only one mutation (A to G, Thr to Ala) was found at 1906bp in this biobrick part(different from E. coli K-12 MG1655). This may have originated from cloning PCR. It is also possible that this is minor mutation of genome of JM109 we used.
  
 
===Source===
 
===Source===

Latest revision as of 19:09, 26 September 2012

fhlA: a transcriptional activator for enhanced hydrogen production


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1375
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Original fhlA gene we cloned from E. coli JM109 has recognition site of PstⅠ.This illegal (inappropriate for biobrick) site was deleted by overlap extension PCR generating mutation (G to A).


Only one mutation (A to G, Thr to Ala) was found at 1906bp in this biobrick part(different from E. coli K-12 MG1655). This may have originated from cloning PCR. It is also possible that this is minor mutation of genome of JM109 we used.

Source

Escherichia coli (JM109)

References