Difference between revisions of "Part:BBa K817007:Design"

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===Design Notes===
 
===Design Notes===
  
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This is the functional testing part we designed to test both the FadR and pfad construct. The FadR is from BBa_K817001.  
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This is the functional testing part we designed to test both the FadR and pfad construct. The FadR is from BBa_K817001. This part included a IPTG-inducible Lac promoter and RBS with FadR gene. FadR protein is a repressor for pfad promoter. When it is expressed and there is no enough fatty acid nearby, this FadR protein will shut down pfad promoter's function. 
  
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This part had also been sequenced and checked. The sequence is identical to the BBa_J04500 and BBa_K817001.
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This part had also been sequenced and checked.
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A few tests had been conducted by 2012 iGEM_Taida. When the ''E. coli'' DH5α were expressing pfad-RBS-mRFP(BBa_K817033), there was a clear red easily seen. After co-transformation with this plac-RBS-FadR construct, the redness decreased. If later you applied oleic acid to the surrounding environment, the redness of the colony once revealed.
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For functional assay result, please refer to our [http://2012.igem.org/Team:NTU-Taida/Result/pFadBA wiki page].
  
 
===Source===
 
===Source===
  
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constitutive promoter-RBS-fadR
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The FadR gene is cloned from ''Escherichia coli'' K12 DH5α genomic DNA. The lac promoter and RBS were from BBa_J04500.
  
 
===References===
 
===References===

Latest revision as of 01:38, 27 September 2012

plac-RBS-FadR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the functional testing part we designed to test both the FadR and pfad construct. The FadR is from BBa_K817001. This part included a IPTG-inducible Lac promoter and RBS with FadR gene. FadR protein is a repressor for pfad promoter. When it is expressed and there is no enough fatty acid nearby, this FadR protein will shut down pfad promoter's function.

This part had also been sequenced and checked.

A few tests had been conducted by 2012 iGEM_Taida. When the E. coli DH5α were expressing pfad-RBS-mRFP(BBa_K817033), there was a clear red easily seen. After co-transformation with this plac-RBS-FadR construct, the redness decreased. If later you applied oleic acid to the surrounding environment, the redness of the colony once revealed.

For functional assay result, please refer to our [http://2012.igem.org/Team:NTU-Taida/Result/pFadBA wiki page].

Source

The FadR gene is cloned from Escherichia coli K12 DH5α genomic DNA. The lac promoter and RBS were from BBa_J04500.

References