Difference between revisions of "Part:BBa K831016"

 
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<partinfo>BBa_K831016 short</partinfo>
 
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Promoter of xpsR (PxpsR) that responds to the Quorum Sensing signal 3 OH-PAME especific of Ralstonia solanacearum.
 
Promoter of xpsR (PxpsR) that responds to the Quorum Sensing signal 3 OH-PAME especific of Ralstonia solanacearum.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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The product of this gene is an integrator signal that regulates the expression of several virulence factors in Ralstonia solanacearum. One of the signals that XpsR integrates is phcA which is involved in the Quorum Sensing mediated by 3 OH-PAME exclusive of'' R. solanacearum''. Therefore expression of genes regulated by phcA like XpsR may be correlated with threshold concentrations of 3 OH-PAME. We designed primers to amplify the upstream region of xpsR gene including the two binding boxes of phcA, the -35 and -10 conserved boxes and excluding the native RBS). This part was confirmed by sequencing.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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Functional confirmation of this part can be found at [https://parts.igem.org/Part:BBa_K831017 BBa:K831017].
 
<partinfo>BBa_K831016 parameters</partinfo>
 
<partinfo>BBa_K831016 parameters</partinfo>
 
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Latest revision as of 15:11, 1 October 2012

PxpsR

Promoter of xpsR (PxpsR) that responds to the Quorum Sensing signal 3 OH-PAME especific of Ralstonia solanacearum.

Usage and Biology

The product of this gene is an integrator signal that regulates the expression of several virulence factors in Ralstonia solanacearum. One of the signals that XpsR integrates is phcA which is involved in the Quorum Sensing mediated by 3 OH-PAME exclusive of R. solanacearum. Therefore expression of genes regulated by phcA like XpsR may be correlated with threshold concentrations of 3 OH-PAME. We designed primers to amplify the upstream region of xpsR gene including the two binding boxes of phcA, the -35 and -10 conserved boxes and excluding the native RBS). This part was confirmed by sequencing. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
    Illegal NgoMIV site found at 312
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

Functional confirmation of this part can be found at BBa:K831017.