Difference between revisions of "Part:BBa K738002:Design"

(New page: __NOTOC__ <partinfo>BBa_K738002 short</partinfo> <partinfo>BBa_K738002 SequenceAndFeatures</partinfo> ===Design Notes=== Generally, RNA strands have a half-life that ranges between 40 s...)
 
(→‎Safety Notes)
 
(3 intermediate revisions by the same user not shown)
Line 7: Line 7:
 
===Design Notes===
 
===Design Notes===
 
Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (''rne''131) gene to limit RNA degradation.
 
Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (''rne''131) gene to limit RNA degradation.
 +
 +
  
 
===Source===
 
===Source===
  
−
Dr. Camille J. Delebecque offer us d0 secquence in plsmid backbone pETDuet-1(Novagan).
+
Clover version 2 was synthesized by ''Genescript''.
  
 
===References===
 
===References===
 
C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, “Organization of intracellular reactions with rationally designed RNA assemblies” ''Science'' 333, 470-474 (2011).
 
C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, “Organization of intracellular reactions with rationally designed RNA assemblies” ''Science'' 333, 470-474 (2011).

Latest revision as of 22:24, 26 September 2012

Theophyline riboswitch regulated RNA Scaffold (clover vision 2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (rne131) gene to limit RNA degradation.


Source

Clover version 2 was synthesized by Genescript.

References

C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, “Organization of intracellular reactions with rationally designed RNA assemblies” Science 333, 470-474 (2011).