Difference between revisions of "Part:BBa K782067"
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* TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011). | * TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011). | ||
| + | * Binding sites for individual TAL effectors are indicated with square brackets [ ]. | ||
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==Introduction== | ==Introduction== | ||
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| − | * Binding sites for TAL effectors were ordered from | + | * Binding sites for TAL effectors were ordered from GeneArt. |
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==Characterization== | ==Characterization== | ||
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Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53. | Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53. | ||
| − | Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698 | + | Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698. |
Latest revision as of 22:50, 26 September 2012
2x[NicTAL]+2x[TALD] operator
- TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
- Binding sites for individual TAL effectors are indicated with square brackets [ ].
Introduction
Our construct contains two specific binding sites for NicTAL12 and TALD.
Single binding sequence for NicTAL12 is: TCTATCAATGATAGA
Single binding sequence for TALD is: TCGTCCAATAGCTTCTC
Figure 1. Shematic representation of two consecutive specific binding sites for NicTAL12 and TALD.
- Binding sites for TAL effectors were ordered from GeneArt.
Characterization
Specific TAL binding sites were further characterized with different reporter constructs.
Results:
References
Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.
Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 35
Illegal AgeI site found at 105 - 1000COMPATIBLE WITH RFC[1000]