Difference between revisions of "Part:BBa K823049:Design"
(→References) |
|||
Line 16: | Line 16: | ||
===References=== | ===References=== | ||
see single BioBricks; [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads] | see single BioBricks; [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads] | ||
+ | |||
+ | [http://www.ncbi.nlm.nih.gov/pubmed/21665972 Imamura ''et la.'', 2011] |
Latest revision as of 09:54, 26 September 2012
PcotYZ - cotZ (-2aa) - gfp - Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 237
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 835
Design Notes
This part is cloned in the B. subtilis pSBBs1C backbone vector for direct integration in the amyE locus of B. subtilis.
Source
genomic B. subtilis, registry (Terminator), Plasmid (pGFPamy)
References
see single BioBricks; [http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins Sporobeads]
[http://www.ncbi.nlm.nih.gov/pubmed/21665972 Imamura et la., 2011]