Difference between revisions of "Part:BBa K266000:Experience"
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There is no data in IPN-UNAM-Mexico iGEM 2009 team’s wiki.  
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Even though a 3OC12HSL-dependent LuxI generator (BBa_K266000) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied. Also, BBa_R0079, the lasI promoter used in BBa_K266000, was evaluated as an ineffective promoter in the Parts Experience page of the Registry. Thus, we constructed a new Plas-LuxI (BBa_K934012) by using an effective lasI promoter (BBa_K649000) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.
  
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We improved this part and confirmed that our part Plas-LuxI ([https://parts.igem.org/Part:BBa_K934012 BBa_K934012]) synthesized enough concentration of 3OC6HSL to induce the Lux reporter cell.
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[[Image:Plas-LuxI_result.png|thumb|center|500px|]]
  
  
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[[Image:Plas-LuxI_result.png|thumb|center|500px|]]
 
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The fluorescence intensity of the Lux reporter cell increased by 112-folds with the induction of 3OC6HSL.
 
  
 
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Latest revision as of 14:34, 26 September 2012


Documentation:The image of the plasmid of this biobrick can be found in [http://2009.igem.org/Image:AI_Biobricks4.jpg (1)] and [http://2009.igem.org/Image:AI_Biobricks4.jpg (2)] with a gel analysis confirming dna size.

Applications of BBa_K266000

In the [http://2009.igem.org/Team:IPN-UNAM-Mexico/Parts IPN-UNAM MEXICO PROJECT]

User Reviews

UNIQ3696e34cc6ad4390-partinfo-00000000-QINU

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Tokyo-tech iGEM 2012

Even though a 3OC12HSL-dependent LuxI generator (BBa_K266000) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied. Also, BBa_R0079, the lasI promoter used in BBa_K266000, was evaluated as an ineffective promoter in the Parts Experience page of the Registry. Thus, we constructed a new Plas-LuxI (BBa_K934012) by using an effective lasI promoter (BBa_K649000) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.

Plas-LuxI result.png


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UNIQ3696e34cc6ad4390-partinfo-00000002-QINU