Difference between revisions of "Part:BBa K769020"

Latest revision as of 07:37, 26 September 2012

lux operon(K769011) + lumP(K769019)

Promoter(Pbad)-lux operon-DT + lumP(K769019)

About Lumazine protein

Bacterial luciferase derived from Vibrio fischeri, which is a strain of luminous bacteria, shows brue-green light emission (wavelength is 495 nm). however, in luminous bacteria, there are strains that emit light with shorter wavelength than normal Vibrio fischeri (475nm,blue), such as Photobacterium phosphoreum and Photobacterium leiognathi. It is known that blue shift of these luminous bacterium is caused by lumazine protein (LumP). LumP shifts the wavelength by interacting with the luciferase. As a chromophore, it has 6,7-dimethyl-8-(1'-D-ribityl)lumazine (DMRL), and its size is about 21kDa.
Learning from previous reports, we decided to use these proteins for changing the color.

The flask on the very right, the brightest strain, is the strain that is expressing both the lux operon and lumP. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
Illegal NheI site found at 1680
Illegal NheI site found at 5955
Illegal NheI site found at 8546
Illegal NheI site found at 8569
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 6417
Illegal BglII site found at 8936
Illegal BamHI site found at 1144
Illegal XhoI site found at 3767
Illegal XhoI site found at 4275
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
Illegal AgeI site found at 1965
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1859
Illegal BsaI.rc site found at 2541
Illegal SapI site found at 961

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-<img src=https://static.igem.org/mediawiki/2012/9/93/Luxoperon.jpg height=80% width=80%>
+https://static.igem.org/mediawiki/2012/7/7f/Luxphoto.jpg
 <BR>
 The flask on the very right, the brightest strain, is the strain that is expressing both the lux operon and lumP.