Difference between revisions of "Part:BBa K769004"

Latest revision as of 02:46, 5 October 2012

NpSRII-9a.a.linker_NpHtrII-EnvZ

This chimeric fusion protein is blue light sensor. This protein is constructed with sensor domain and kinase domain. Sensor domain is sensory rhodopsin II (SRII), which fused by a flexible linker to transducer, halobacterium transducer protein II (HtrII). SRII is a repellent receptor sensing blue light (λ max 487 nm). SRII interacts with HtrII, which in turn controls the activity of a histidine kinase. Histidine kinase we used is the kinase domain of EnvZ. EnvZ phosphorylateｓ response OmpR, which is regulator of two-compoment system. Phosphorylated OmpR activates OmpC promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 73
Illegal NgoMIV site found at 331
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1027
Illegal SapI site found at 801

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 <partinfo>BBa_K769004 short</partinfo>
-NpSRII-9a.a.linker_NpHtrII-EnvZ
+This chimeric fusion protein is blue light sensor. This protein is constructed with sensor domain and kinase domain. Sensor domain is sensory rhodopsin II (SRII), which fused by a flexible linker to transducer, halobacterium transducer protein II (HtrII). SRII is a repellent receptor sensing blue light (λ max 487 nm). SRII interacts with HtrII, which in turn controls the activity of a histidine kinase. Histidine kinase we used is the kinase domain of EnvZ. EnvZ phosphorylateｓ response OmpR, which is regulator of two-compoment system. Phosphorylated OmpR activates OmpC promoter.
 <BR>https://static.igem.org/mediawiki/parts/8/84/Safety.png
 <!-- Add more about the biology of this part here