Difference between revisions of "Part:BBa K737034"
 
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Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one.
 
Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one.
 
https://static.igem.org/mediawiki/parts/7/78/4-1.PNG
 
https://static.igem.org/mediawiki/parts/7/78/4-1.PNG
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https://static.igem.org/mediawiki/parts/0/0b/Gmh1.PNG
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The pairing hybridization Energy is -19.2 kcal/mol. 18 bases participate in the pairing. However only 2.6-fold repression is presented and not significantly dependence on E.coli hfq
  
 
===Experimental Data===
 
===Experimental Data===

Latest revision as of 13:13, 26 September 2012

galK::GFP generator

Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one. 4-1.PNG

Gmh1.PNG

The pairing hybridization Energy is -19.2 kcal/mol. 18 bases participate in the pairing. However only 2.6-fold repression is presented and not significantly dependence on E.coli hfq

Experimental Data

galK::GFP_Generator without expressing Spot42 could be repressed by IPTG/aTc in an unclear way. One assumption is that the cytotoxicity of inducers impairs the capacity of expressing protein.

ZPR.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 819