Difference between revisions of "Part:BBa K818600"

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<partinfo>BBa_K818600 short</partinfo>
 
<partinfo>BBa_K818600 short</partinfo>
  
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This is a coding construct for ''B. subtilis'' to produce AmilGFP pigment in response to rotten meat volatiles in the environment. AmilGFP is a yellow pigment designed by iGEM Uppsala 2011 and the regulator, sboA, is designed by iGEM Groningen 2012.  
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This is a coding construct for ''B. subtilis'' to produce AmilGFP pigment in response to spoiled meat volatiles in the environment. AmilGFP is a yellow pigment designed by iGEM Uppsala 2011 and the regulator, sboA, is designed by iGEM Groningen 2012.  
  
 
===Usage and Biology===
 
===Usage and Biology===
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Check out the data sheet below for more information - or click [https://static.igem.org/mediawiki/parts/5/50/RR_Groningen_818600_Data_sheet.pdf here] to download the pdf.
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[[Image:RR_Groningen_Data_sheet_Page_1.png|400px|left]]
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[[Image:RR_Groningen_Data_sheet_Page_2.png|400px|right]]
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'''Expression in ''E. coli'''''
 
  
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''SboA-AmilGFP'' is strongly expressed in ''E. coli'', on plate in liquid culture, at normal growth conditions. On plate, the construct is expressed less strongly.
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K818600 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K818600 SequenceAndFeatures</partinfo>
  

Latest revision as of 03:31, 27 October 2012

AmilGFP with sboA regulator

This is a coding construct for B. subtilis to produce AmilGFP pigment in response to spoiled meat volatiles in the environment. AmilGFP is a yellow pigment designed by iGEM Uppsala 2011 and the regulator, sboA, is designed by iGEM Groningen 2012.

Usage and Biology

Check out the data sheet below for more information - or click here to download the pdf.

RR Groningen Data sheet Page 1.png
RR Groningen Data sheet Page 2.png

























Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]