Difference between revisions of "Part:BBa K864403"
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<partinfo>BBa_K864403 short</partinfo> | <partinfo>BBa_K864403 short</partinfo> | ||
| − | This part | + | This part is a coding device for the chromoprotein aeBlue (<partinfo>BBa_K864401</partinfo>) by means of promoter <partinfo>BBa_J23110</partinfo> and the RBS <partinfo>BBa_B0034</partinfo>. |
| − | aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone ''Actinia equina''. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities. | + | aeBlue (more info on <partinfo>BBa_K864401</partinfo>) is a blue chromoprotein extracted from the basal disk of a beadlet anemone ''Actinia equina''. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities. |
| − | Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654. | + | |
| + | [[Image:IMG 4371a.jpg|400px]] | ||
| + | [[Image:AeBlue_onplate.jpg|400px]] | ||
| + | |||
| + | ===References=== | ||
| + | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/]Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | This part is useful for visual screening and for example be used instead of can be used as | |
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Latest revision as of 23:57, 25 September 2012
J23110-B0034-aeBlue (chromoprotein) (E.coli optimized)
This part is a coding device for the chromoprotein aeBlue (BBa_K864401) by means of promoter BBa_J23110 and the RBS BBa_B0034.
aeBlue (more info on BBa_K864401) is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.
References
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316306/]Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]