Difference between revisions of "Part:BBa K819016:Design"

Latest revision as of 09:34, 26 September 2012

Luminesensor repressible SulA408 promoter+CheZ

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

CheZ coding sequence was placed under SulA408 promoter (BBa_K819017).

Source

CheZ was extracted from E.Coli strain MG1655 by colony PCR.
SulA408 promoter was from BBa_K819017

References

1.Scotoc. Kuo and Daniele. Koshland,JR.(1987) Roles of che Y and cheZ Gene Products in Controlling Flagellar Rotation in Bacterial Chemotaxis of Escherichia coli. J. Bacteriol., 3:1307:1313
2.M. Germana Sanna and Melvin I. Simon(1996) In Vivo and In Vitro Characterization of Escherichia coli Protein CheZ Gain- and Loss-of-Function Mutants. J. Bacteriol., 178: 6275: 6280

@@ Line 6: / Line 6: @@
 ===Design Notes===
-CheZ coding sequence ligated to the Luminesensor repressible SulA408 promoter.
+CheZ coding sequence was placed under SulA408 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K819017 BBa_K819017]).
 ===Source===
-CheZ was extracted from E.Coli strain MG1655 by colony PCR.
+CheZ was extracted from E.Coli strain MG1655 by colony PCR.<br />SulA408 promoter was from [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819017 BBa_K819017]
-SulA promoter was from [https://parts.igem.org/wiki/index.php?title=Part:BBa_K819017 BBa_K819017]
 ===References===
+.Scotoc. Kuo and Daniele. Koshland,JR.(1987) Roles of che Y and cheZ Gene Products in Controlling Flagellar Rotation in Bacterial Chemotaxis of Escherichia coli. J. Bacteriol., 3:1307:1313<br />
+.M. Germana Sanna and Melvin I. Simon(1996) In Vivo and In Vitro Characterization of Escherichia coli Protein CheZ Gain- and Loss-of-Function Mutants. J. Bacteriol., 178: 6275: 6280<br />